anti kitl  (R&D Systems)

Journal: Biomolecules

Article Title: Evidence for FOXL2 Association with the Tsc1 Regulatory Region in Mice

doi: 10.3390/biom16040510

Figure Lengend Snippet: Expression and localization of TSC1/FOXL2 and KITL/VASA1 in P7 mouse ovaries. ( a ) Immunofluorescence was carried out in sections of P7 Foxl2 WT mouse ovary, using anti-FOXL2 and anti-TSC1 antibodies. FOXL2 is expressed in the GCs of primordial and primary follicles but not in the oocytes. TSC1 is expressed both in the GCs and in the oocytes of maturating follicles. The yellow box highlights the zoomed-in region showing co-localisation at the cell level. ( b ) Immunofluorescence was carried out in sections of P7 Foxl2 WT and Foxl2 −/− mouse ovaries, using anti-KITL and anti-VASA1 antibodies. In blue, nuclear counterstaining performed with DAPI. Immunofluorescence imaging was acquired using a Leica DMIRE2-TCS-SL Confocal Laser Scanning microscope and with a HiRes CCD camera and processed on a Deltavision system version 5.10. Magnification bars indicate 50 μm at 20× and 63×, 10 μm at 100×.

Article Snippet: The antibodies used were anti-FOXL2 (#ab5096, 1:25, Abcam, Cambridge, UK), anti-TSC1 (#MBS176028, 1:200, MyBioSource, San Diego, CA, USA), anti-KITL (#bs-0545R, 1:200, Bioss, Woburn, MA, USA), and anti-VASA ((#A21206, BD Pharmingen 560189, 1:100).

Techniques: Expressing, Immunofluorescence, Imaging, Laser-Scanning Microscopy

Journal: medRxiv

Article Title: Expression of mechano-growth factor (MGF) in refractory overactive bladder

doi: 10.1101/2023.12.08.23299594

Figure Lengend Snippet: An antibody specific to the MGF E-peptide was used to detect expression of MGF protein by immunohistochemistry staining (a) and western blot (b-d) analysis of biopsy sections. In (a) 5µm sections of formalin-fixed OAB bladder biopsies were stained with anti-MGF (i) or secondary antibody only control (ii) and counterstained with haematoxylin. In (b-d) 30µg biopsy lysates were analysed by western blot for detection of MGF (∼15 kDa) and β-actin (42 kDa) as a loading control. L=size ladder. A representative blot is shown in (b) and a scatter plot showing quantification of MGF band intensity is shown in (c). The bar indicates mean relative MGF expression in control and OAB biopsies (0.22 and 0.51, respectively). In (d), the graph shows mean relative MGF expression +/- SEM in control/OAB biopsies in the three main sub-groups identified in : DES hi (n=6/5), VIM hi (n=1/6), DES&VIM hi (n=2/6). Statistical analysis in (c) and (d) was performed using the Mann-Whitney U test.

Article Snippet: Sections were stained using an anti-MGF E-peptide antibody (Merck Life Sciences #07-2108, rabbit polyclonal, 1/50 dilution) and HRP-tagged secondary (Vector Laboratories, BA-1100, 1/200 dilution), or secondary only control staining.

Techniques: Expressing, Immunohistochemistry, Staining, Western Blot, MANN-WHITNEY